■ Vave ma maualuga fua: 200 μg-1.5 mg plasmid DNA fua i le 1 itula ma maualuga vaega o le maoaʻe-coiled fausaga.
■ Maualuga-mama: Maualuga-mama plasmid DNA e maua e le faʻapitoa buffer system ma Spin Column CP6.
■ Lelei tele transfection lelei: Fetaui lelei mo transfection tofotofoga o le tele sela laina.
■ Tele lautele o talosaga: E talafeagai mo le faʻatapulaʻaina o le enzyme digestion, fesuiaʻiga, faʻasologa, microinjection, faʻafilemuina o genes ma transfection faʻataʻitaʻiga.
Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)
Maualuga / maualalo kopi aveʻesea mai plasmid Low-copy plasmid pBR322, aveese mai le 200 ml siama siama faʻaaoga ai le EndoFree Maxi Plasmid Kit, na faʻaalia i le 1 ml Buffer TB, faatasi ai ma le aofaiga o 0.6 μg / μl. Maualuga-kopi plasmid pBS, aumai mai le 100 ml siama siama faʻaaogaina o le EndoFree Maxi Plasmid Kit, na faʻaalia i le 1 ml Buffer TB, ma le faʻatosina o le 1.2 μg / μl. MIV: TIANGEN DNA Faailoga IV pBR322: 2 μl, pBS: 2 μl |
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O le pEGFP na mauaina ma le EndoFree Maxi Plasmid Kit na eseʻese ese atu i totonu o le endotoxin-insensitive cell laina 293T ma le endotoxin-sensitive cell laina Huh7 sela e TIANGEN TIANfect. O le faʻaalia o le GFP na maitauina i le 24 itula talu ona tui. |
Talu mai lona faavaeina, o lo tatou fale gaosi ua atiina ae muamua lalolagi vasega oloa ma mulimulitaia le mataupu faavae
o lelei muamua. Tatou oloa ua maua sili lelei taʻuleleia i le alamanuia ma valuabletrusty i fou ma tuai tagata faʻatau ..