TGuide Bacteria Genomic DNA Pusa

Mo le aveʻese mai genomic DNA mai siama.

TGuide Bacterial Genomic DNA Kit e faʻapitoa lava ona faʻamamaina e faʻamamaina le genomic DNA mai le siama o le Gram-leaga ma siama Gram-positive e faʻaaogaina ai le TGuide Series Automated Nucleic Acid Extractor. E mafai foi ona faʻaaogaina mo le aveʻese mai o genome o siama pathogenic bacteria (microorganisms), pei o Staphylococcus aureus, Vibrio cholerae, hemorrhagic Escherichia coli O157: H7, Listeria monocytogenes, Salmonella, Enterobacter sakazakii,ma isi mea o loʻo i totonu o le pusa meaola ma mea faʻaaoga manaʻomia mo otometi DNA toʻesea mai e maneta faiga lopa. O faʻamalositino o loʻo teuina muamua i totonu o faʻamau faʻailoga vaʻaia. O le ese tulaga ofuina manoa manoa ma atoa otometi toeseina gaioiga mautinoa le vave ma talafeagai DNA faamamaina.

Pusi leai Tele teuina
OSR-M502 48 preps

Oloa Faʻamatalaga

Faʻataʻitaʻiga Faʻataʻitaʻiga

FAQ

Pine o oloa

Talosaga

O le genomic DNA faʻamamaina e mafai ona faʻaaoga saʻo mo le tele o le PCR, faʻatapulaʻaina o le enzyme digestion, Southern hybridization ma isi faʻataʻitaʻiga.

Features

■ Faigofie ma vave aveʻeseʻesega: TGuide oloa fesoasoani o loʻo faʻavae i luga o le faʻavae o le nucleic acid faʻamamaina e manoa manoa, ma le genomic DNA faʻagasolo gaioiga mafai ona maeʻa i le 44 min.
■ lautele faʻaaogaina: E mafai ona aumaia genomic DNA o kalama-leaga siama ma siama-lelei siama, ma mafai foi ona faʻaaogaina mo le aumaia o genomic DNA o meaʻai pathogenic siama (microorganisms).
■ Leai se phenol ma se chloroform e tosoina i fafo: Leai ni vailaʻau e mafai ona afaina ai le tino o le tagata pei o le phenol ma le chloroform e manaʻomia.

Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)


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    Experimental Example Genomic DNA na aumaia mai siama (Bacillus subtilis) Faʻamalologa o vaega eseese.
    DNA ua faʻamamaina i le 100 μl elution buffer. Utaina voluma: 5 μl
    Faʻataʻitaʻiga 1: 0.25 ml;
    Faʻataʻitaʻiga 2: 0.5 ml;
    Faʻataʻitaʻiga 3: 0.75 ml;
    Faʻataʻitaʻiga 4: 1 ml;
    Faʻataʻitaʻiga 5: 2 ml.
    Experimental Example Siama genomic DNA na aumaia mai faʻavae eseese. DNA na faʻamamaina i le 100 μl elution buffer. Utaina voluma: 5 μl
    1: E. coli DH5α
    2: Bacillus subtilis
    Q: Laʻititi pe leai foi DNA i le eluent.

    A-1 Maualalo le totoina o sela poʻo siama i le amataga o le faʻataʻitaʻiga - Faʻamalosia le faʻasologa o sela poʻo siama.

    A-2 Lysis le lava o faʻataʻitaʻiga - O faʻataʻitaʻiga e leʻi maeʻa maeʻa faʻafefiloi ma le buffer lysis. E fautuaina e fefiloi maeʻa ile pulse-vortexing mo le 1-2 taimi. —Ua le lava le sela o le sela na mafua mai i le faʻaititia o proteinase K. - Le lava le lysis sela poʻo le faʻaleagaina o le polotini ona o le le lava o le vai tavela mafanafana. E fautuaina e tipi le fasi pepa i ni fasi laiti ma faʻalautele le taimi taele e aveʻese uma toega i le lysate.

    A-3 Le lava faʻasalalauga DNA. —E leai se ethanol poʻo se maualalo pasene nai lo le 100% ethanol na faʻaopopoina ae le i faʻamatuʻu le lysate i le vilivalavala.

    A-4 O le taua ole pH ole faʻaola eletise e maualalo tele. —Faiaso le pH i le va o le 8.0-8.3.

    Q: E leʻo lelei le faʻagaioiga ole DNA ile faʻataʻitaʻiga ole faʻasolosolo ole enzymatic.

    Ethanol totoe i le eluent.

    —E i ai toega fufuluina PW i le eluent. O le ethanol e mafai ona aveʻese i le centrifuging le vili pou mo 3-5 min, ona tuʻu ai lea i le potu vevela poʻo le 50 ℃ incubator mo 1-2 min.

    Q: DNA faʻaleagaina

    A-1 O le faʻataʻitaʻiga e le fou. — Aveʻese se faʻataʻitaʻiga lelei o le DNA e fai ma faʻatonutonu e faʻamautinoa ai pe ua faʻaleagaina le DNA i totonu o le faʻataʻitaʻiga.

    A-2 Leaga muamua togafitiga. —Faʻailoaina e le soʻona suavaia oona nitrogen, toe maua le susu, poʻo tele tele o le faʻataʻitaʻiga.

    Q: Faʻafefea ona faʻatinoina le muaʻi faʻaaliga mo le aveʻeseina o le gDNA?

    E tatau ona fesuisuiaʻi mea e fai mo faʻataʻitaʻiga eseese. Mo faʻataʻitaʻiga laʻau, ia mautinoa ia olo lelei i le nitrogen suavaia. Mo manu faʻataʻitaʻiga, homogenate poʻo olo lelei i le suavaia oona. Mo faʻataʻitaʻiga ma sela sela e faigata ona gagau, pei o le G + bacteria ma le fefete, e fautuaina e faʻaoga le lysozyme, lyticase poʻo metotia e motusia ai sela o sela.

    Q: O le a le eseesega i le va o le tolu laau gDNA aveʻesea pusa 4992201/4992202, 4992724/4992725, 4992709/4992710?

    4992201/4992202 Laʻau Genomic DNA Kit faʻaaogaina se koluma-faʻavae metotia e manaʻomia chloroform mo le aveʻesea. E sili ona talafeagai mo eseese fualaʻau faʻataʻitaʻi, faʻapea foi ma laʻau mago paʻu. O le Hi-DNAsecure Plant Kit o loʻo faʻavaeina foi i luga ole koluma, ae leai se manaʻoga o le faʻateʻaina o le phenol / chloroform, e saogalemu ai ma e le oona foi. E fetaui lelei mo laʻau ma maualuga polysaccharides ma polyphenol mea. 4992709/4992710 DNAquick Plant System faʻaogaina se vai-faʻavae metotia. Phenol / chloroform aveʻesea e le manaʻomia foi. O le faʻamamaina gaioiga e faigofie ma vave ma leai se tapulaʻa mo le amataga amataga aofaʻiga, o lea e mafai ai e tagata faʻaoga ona fetuʻunaʻi le aofaʻi fetuʻunaʻi e tusa ai ma faʻataʻitaʻiga manaʻoga. Tele lapoa o gDNA fasi e mafai ona maua ma maualuga fua.

    O le a le faʻatatauina maua o gDNA mai le 1 ml toto faʻataʻitaʻiga e TIANamp Toto DNA Pusa?

    O le genomic DNA na aumaia mai vaega eseese o faʻataʻitaʻiga o le toto o le tagata e le TIANamp Blood DNA Kit. O iʻuga e mulimuli mai. O iʻuga e lisiina naʻo faʻasino, o le mea moni toʻeseʻesea iʻuga e faʻamoemoeina i tuutuuga o faʻataʻitaʻiga.

    faq

    F: Mafai e le 4992207/4992208 ma le 4992722/4992723 ona faʻaaogaina e aveʻesea ai le toto masaa o le DNA?

    E mafai ona faʻatino le vailaʻau toto DNA e faʻaaoga ai meaʻai na saunia i nei mea e lua i le naʻo le suia o le maliega faʻapitoa i faʻatonuga faʻapitoa mo le aveʻesea o le toto o le DNA. O le kopi vaivai o le toto clot DNA aveʻesega maliega maliega faʻapitoa mafai ona tuuina atu pe a talosagaina.

    F: Pe a faʻaogaina le TIANamp Genomic DNA Kit, faʻafefea ona gagau ia mea fou i totonu o le sela taofia?

    Taofi le faʻataʻitaʻiga fou ile 1 ml PBS, saline masani poʻo le buffer TE. Faʻamaeʻa uma homogenize le faʻataʻitaʻiga e se homogenizer ma ao le oso i lalo o le paipa e centrifuging. Tuʻu ese le supernatant, ma resuspend le oso i le 200 μl buffer GA. Ole faʻamamaina ole DNA e mafai ona faia e tusa ma faʻatonuga.

    Q: Faʻafefea ona filifili le oloa mo le DNA toʻesea mai plasma, serum ma tino faʻataʻitaʻiga suavai?

    Mo le faʻamamaina o gDNA i plasma, serum ma tino faʻataʻitaʻiga suavai, TIANamp Micro DNA Pusa ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum / faʻataʻitaʻiga plasma, TIANamp Virus DNA / RNA Kit ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum ma faʻataʻitaʻiga plasma, TIANamp Bacteria DNA Pusa ua fautuaina (lysozyme tatau ona aofia ai mo lelei siama). Mo faʻataʻitaʻiga faua, Hi-Swab DNA Pusa ma TIANamp Bacteria DNA Pusa ua fautuaina.

    Q: Faʻafefea ona filifili ato mo le aveʻesea o le gDNA mai faʻataʻitaʻiga mai le fungi?

    DNAsecure Plant Pusa poʻo DNAquick Plant System e fautuaina mo le aveʻese mai o le genome. Mo le toʻafilemuina o le genome, TIANamp Yeast DNA Kit e fautuaina (lyticase tatau ona saunia e ia lava).

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