TIANprep Rapid N96 Plasmid Pusa

Maualuga maualuga, vave aveʻese mai laʻititi aofaʻi o plasmid.

O le pusa faʻaaogaina le isopropanol faʻapipiʻi metotia e maua ai plasmid DNA. O le pusa e faʻaaogaina le 96-lelei faʻamama ipu ipu N ma Buffer III, o loʻo faʻapitoa atinaeina e TIANGEN, ma mafai ona vave ma lelei maua mai maualuga-mama plasmid DNA. E talafeagai mo vave sauniuniga o maualuga-throughput plasmids. 1.3 ml o le maualuga-kopi plasmid siama siama aganuʻu mafai ona gaioiga i le vaieli lelei, ma 96 faʻataʻitaʻiga o le plasmid DNA e vave maua mai. Ole fua maualuga o le vai e tasi o le 10-20 μg (o le fua e fesoʻotaʻi ma mea taua e pei o le plasmid kopi numera, siama tulaga, ma isi). E mafai ona faʻaaogaina le Plasmid DNA na aumaia e le pusa i faʻataʻitaʻiga o meaola o le paiolo e pei o le faʻatapulaʻaina o le enzyme digestion, faʻasologa, faʻataʻitaʻiga o le faletusi, ligation ma suiga.