EndoFree Maxi Plasmid Pusa

Mo le faʻamamaina o endotoxin-leai se fesuiaʻiga vasega plasmid DNA.

O le EndoFree Maxi Plasmid Kit e faʻavae i luga o le silica membrane technology e aumaia ai le endotoxin-free plasmid DNA. Lenei pusa e maua ai se tulaga ese endotoxin aveʻese gaioiga e gaosia maualuga-lelei plasmid DNA mo lalo auala. Ua fuafuaina e tuʻu eseʻese le plasmid DNA o le maualuga mama mai le 100-200 ml o siama siama ma mafai ai ona oʻo atu i le 1.5 mg transfection vasega plasmid DNA.

Pusi leai Tele teuina
4992194 10 preps

Oloa Faʻamatalaga

Faʻataʻitaʻiga Faʻataʻitaʻiga

Pine o oloa

Features

■ Vave ma maualuga fua: 200 μg-1.5 mg plasmid DNA fua i le 1 itula ma maualuga vaega o le maoaʻe-coiled fausaga.
■ Maualuga-mama: Maualuga-mama plasmid DNA e maua e le faʻapitoa buffer system ma Spin Column CP6.
■ Lelei tele transfection lelei: Fetaui lelei mo transfection tofotofoga o le tele sela laina.
■ Tele lautele o talosaga: E talafeagai mo le faʻatapulaʻaina o le enzyme digestion, fesuiaʻiga, faʻasologa, microinjection, faʻafilemuina o genes ma transfection faʻataʻitaʻiga.

Fuafua Plasmid

Plasmid Yield

Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)


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    Experimental Example Maualuga / maualalo kopi aveʻesea mai plasmid
    Low-copy plasmid pBR322, aveese mai le 200 ml siama siama faʻaaoga ai le EndoFree Maxi Plasmid Kit, na faʻaalia i le 1 ml Buffer TB, faatasi ai ma le aofaiga o 0.6 μg / μl. Maualuga-kopi plasmid pBS, aumai mai le 100 ml siama siama faʻaaogaina o le EndoFree Maxi Plasmid Kit, na faʻaalia i le 1 ml Buffer TB, ma le faʻatosina o le 1.2 μg / μl.
    MIV: TIANGEN DNA Faailoga IV
    pBR322: 2 μl, pBS: 2 μl
    Experimental Example O le pEGFP na mauaina ma le EndoFree Maxi Plasmid Kit na eseʻese ese atu i totonu o le endotoxin-insensitive cell laina 293T ma le endotoxin-sensitive cell laina Huh7 sela e TIANGEN TIANfect. O le faʻaalia o le GFP na maitauina i le 24 itula talu ona tui.
    Tusi lau feʻau ii ma lafo mai ia matou