DNA Bisulfite Faʻaliliuga Pusa

O le liuaina ma le faʻamamaina mafai ona maeʻa i le 2 itula, ma le liua fua faatatau oʻo i le 99%.

DNA Bisulfite Liua Pusa e faʻapitoa mo le DNA bisulfite liua i DNA methylation suʻesuʻega. O le togafitiga o DNA faʻataʻitaʻiga faʻaaogaina lenei pusa e mafai ona maeʻa i le 2 itula, ma o le liua fua faatatau o unmethylated cytosine i uracil e sili atu i le 99%. Lenei pusa faʻaaogaina se vaega tulaga ese o DNA puipuiga sooupu, lea e mautinoa ai le lelei ma le toe faʻaleleia o DNA pe a uma le liua. I se faʻaopopoga, o lenei pusa faʻaaoga foi le metotia o luga o le koluma sulphurous acid vaega aveʻesea, lea e faʻafaigofie ai le atoa faʻagaioiga gaioiga.

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Features

■ Faigofie ma vave: O le liua ma le faʻamamaina gaioiga mafai ona maeʻa i le 2 itula, ma maualalo manaʻoga mo mea faigaluega ma masini, lea e talafeagai mo suʻesuʻega faʻalapotopotoga i uma tulaga.
■ Fua faatatau maualuga o le liuaina: O le liua fua faatatau o unmethylated cytosine i uracil i DNA faʻataʻitaʻiga e sili atu i le 99%.
■ Maualuga le malamalama: O lenei pusa e mafai ona faʻagasolo DNA faʻataʻitaʻiga o le maualalo o le 500 pg ma oʻo atu i le 2.5 μg.

Faʻamatalaga

Ituaiga:  Le Liua o le Bisulfite
DNA faʻataʻitaʻiga: 500 iai-2.5 μg
Le fua faatatau o le liua: > 99%
Taimi o galuega: ~ 2 itula
Talosaga: O DNA faʻataʻitaʻiga faʻataʻitaʻia e lenei pusa e talafeagai mo methylation faʻapitoa PCR / qPCR, faʻasologa ma microarray.

Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)


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    Lisi 1: Fuafua le suiga o le "C" unmethylated i le "U"
    I le faʻaaogaina o le genome o le tagata e avea o ni mea faʻataʻitaʻi, o le toe maua mai o le faʻataʻitaʻiga e mafai ona oʻo atu i le 65%, ma le fua faatatau o le unmethylated "C" i le "U" e mafai ona oʻo atu i le 99%.

    Table 1: Conversion rate of unmethylated “C” to “U” Using human genome as experimental material, the recovery rate of the sample can reach 65%, and the ratio of unmethylated “C” to “U” can reach more than 99%.

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