HRM Suʻesuʻega Pusa (EvaGreen)

Features

■ Maualuga iugafono: Faʻaaoga EvaGreen vali vali, lea e maualuga le fofo o le liʻo liʻo i le totoa tulaga ma mafai ona iloa le eseesega o le tasi faʻavae.
■ Maualuga faʻapitoa: Faʻaoga le vailaʻau faʻasaina o le hotstart DNA polymerase e faʻaititia ai le le faʻapitoa o le faʻalautelega ma faʻaleleia atili ai le faʻapitoa.
■ Maualuga maualuga: O le faʻaeteete faʻalelei sisitema sisitema faʻateleina le mautu o le liusuavai piʻo ma faʻaleleia le faʻatuatuaina o iʻuga.
■ Faʻasaʻoga ROX: O le ROX vali e tuʻufaʻatasia afifi, e sili atu fetuʻutuʻunaʻi e faʻaoga ma maua ai isi faʻamatalaga saʻo.

Faʻamatalaga

Ituaiga: Antibody fesuiaʻi Taq DNA polymerase, EvaGreen.
Talosaga: Iloa SNP taina; Le faʻailoaina SNP faʻailoaina; Suʻesuʻe mutes genes suʻesuʻeina; Methylation PCR auiliiliga.

Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)

×

O le Genomic DNA na aumaia mai le 100 μl o faʻataʻitaʻiga o le toto o le tagata e faʻaaogaina ai le TIANamp Blood DNA Kit. 50 ng DNA na utaina mo le taimi moni mauaina PCR. E tusa ai ma le faletusi a le NCBI SNP, o le SNP lauiloa o le FEN1 gene (RS 174538) ua filifilia ma vaʻaia e faʻaaogaina ai Roche LightCycle480. O iʻuga nei:

O iʻuga ua faʻaalia ai o le HRM Analysis Kit o se metotia sili ona lelei SNP locus analysis metotia ma le maualuga faʻafouina o le liʻo liʻo o le tutusa genotype, manino iugafono o genotypes 'eseʻese ma leai se faʻaseseina.

Faamatalaga SNP: …… CGGAAGAACACGTCG [A / G] CAGGAGCAGGCGCCT …… (Allele Frequency: A = 0.314, G = 0.686)

Mea muamua: Mo 108 bp fasi (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTTCCGGTTGTG)