TGuide FFPE DNA Tasi Sitepu
Talosaga
Ole DNA faʻamamaina e mafai ona faʻaaoga saʻo ile faʻagaioiga masani, e iai ma le digestion o le enzymatic, PCR, fausiaina ole faletusi, South blot ma isi faʻataʻitaʻiga.
Features
■ Faʻalumaina o masini i totonu o le masini: O le faʻaaogaina o le tino ma le aveeseina o laʻasaga e mafai ona faʻamaeʻaina otometi i le na o le tuʻuina o faʻataʻitaʻiga o le paraffin ua uma ona faʻapipiʻi i totonu o le katuni faʻaalu ma faʻaopopo Sula Suauʻu ma Proteinase K.
■ Faʻamoemoeina taunuʻuga: O le mauaina genome DNA e leai se mea mai le RNA ma le faʻaleagaina o polotini, ma e mafai ona faʻaaoga saʻo lava mo PCR poʻo fluorescence aofaʻi PCR.
■ Saogalēmū ma leai se afaina: O soli totoʻo e afaina ai le tino o le tagata pei o le phenol chloroform e le manaʻomia.
Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)
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O iʻuga na faʻaalia ai, mo tele lapoʻa faʻataʻitaʻiga, o le toʻesea mai o mea e lua o metotia e faʻatusatusa; mo faʻataʻitaʻiga ma feololo ma laiti tusi, o le fua o le tasi laasaga deparaffinization metotia (faatino ma TGuide) na sili atu nai lo le masani deparaffinization metotia. O le mea lea, o le tasi laasaga deparaffinization metotia e le gata faʻafaigofieina le faʻagaioiga, faʻaititia ai le aʻafiaga, ae faʻaleleia atili ai foi le aveʻesega lelei. Faʻaaliga: Tele voluma faʻataʻitaʻiga: o le vaega vaeluaina o totonu o 2.5-4 cm2 ma le mafiafia o totonu o 5-20 μm. Feololo feololo faʻataʻitaʻiga: o le vaevaega vaega o totonu o 1.5-2.5 cm2 ma le mafiafia o totonu o 5-20 μm. Laʻititi voluma faʻataʻitaʻiga: o le vaega vaeluaina o totonu o 0.2-1.5 cm2 ma le mafiafia o totonu o 5-20 μm. |
A-1 Maualalo le totoina o sela poʻo siama i le amataga o le faʻataʻitaʻiga - Faʻamalosia le faʻasologa o sela poʻo siama.
A-2 Lysis le lava o faʻataʻitaʻiga - O faʻataʻitaʻiga e leʻi maeʻa maeʻa faʻafefiloi ma le buffer lysis. E fautuaina e fefiloi maeʻa ile pulse-vortexing mo le 1-2 taimi. —Ua le lava le sela o le sela na mafua mai i le faʻaititia o proteinase K. - Le lava le lysis sela poʻo le faʻaleagaina o le polotini ona o le le lava o le vai tavela mafanafana. E fautuaina e tipi le fasi pepa i ni fasi laiti ma faʻalautele le taimi taele e aveʻese uma toega i le lysate.
A-3 Le lava faʻasalalauga DNA. —E leai se ethanol poʻo se maualalo pasene nai lo le 100% ethanol na faʻaopopoina ae le i faʻamatuʻu le lysate i le vilivalavala.
A-4 O le taua ole pH ole faʻaola eletise e maualalo tele. —Faiaso le pH i le va o le 8.0-8.3.
Ethanol totoe i le eluent.
—E i ai toega fufuluina PW i le eluent. O le ethanol e mafai ona aveʻese i le centrifuging le vili pou mo 3-5 min, ona tuʻu ai lea i le potu vevela poʻo le 50 ℃ incubator mo 1-2 min.
A-1 O le faʻataʻitaʻiga e le fou. — Aveʻese se faʻataʻitaʻiga lelei o le DNA e fai ma faʻatonutonu e faʻamautinoa ai pe ua faʻaleagaina le DNA i totonu o le faʻataʻitaʻiga.
A-2 Leaga muamua togafitiga. —Faʻailoaina e le soʻona suavaia oona nitrogen, toe maua le susu, poʻo tele tele o le faʻataʻitaʻiga.
E tatau ona fesuisuiaʻi mea e fai mo faʻataʻitaʻiga eseese. Mo faʻataʻitaʻiga laʻau, ia mautinoa ia olo lelei i le nitrogen suavaia. Mo manu faʻataʻitaʻiga, homogenate poʻo olo lelei i le suavaia oona. Mo faʻataʻitaʻiga ma sela sela e faigata ona gagau, pei o le G + bacteria ma le fefete, e fautuaina e faʻaoga le lysozyme, lyticase poʻo metotia e motusia ai sela o sela.
4992201/4992202 Laʻau Genomic DNA Kit faʻaaogaina se koluma-faʻavae metotia e manaʻomia chloroform mo le aveʻesea. E sili ona talafeagai mo eseese fualaʻau faʻataʻitaʻi, faʻapea foi ma laʻau mago paʻu. O le Hi-DNAsecure Plant Kit o loʻo faʻavaeina foi i luga ole koluma, ae leai se manaʻoga o le faʻateʻaina o le phenol / chloroform, e saogalemu ai ma e le oona foi. E fetaui lelei mo laʻau ma maualuga polysaccharides ma polyphenol mea. 4992709/4992710 DNAquick Plant System faʻaogaina se vai-faʻavae metotia. Phenol / chloroform aveʻesea e le manaʻomia foi. O le faʻamamaina gaioiga e faigofie ma vave ma leai se tapulaʻa mo le amataga amataga aofaʻiga, o lea e mafai ai e tagata faʻaoga ona fetuʻunaʻi le aofaʻi fetuʻunaʻi e tusa ai ma faʻataʻitaʻiga manaʻoga. Tele lapoa o gDNA fasi e mafai ona maua ma maualuga fua.
O le genomic DNA na aumaia mai vaega eseese o faʻataʻitaʻiga o le toto o le tagata e le TIANamp Blood DNA Kit. O iʻuga e mulimuli mai. O iʻuga e lisiina naʻo faʻasino, o le mea moni toʻeseʻesea iʻuga e faʻamoemoeina i tuutuuga o faʻataʻitaʻiga.
E mafai ona faʻatino le vailaʻau toto DNA e faʻaaoga ai meaʻai na saunia i nei mea e lua i le naʻo le suia o le maliega faʻapitoa i faʻatonuga faʻapitoa mo le aveʻesea o le toto o le DNA. O le kopi vaivai o le toto clot DNA aveʻesega maliega maliega faʻapitoa mafai ona tuuina atu pe a talosagaina.
Taofi le faʻataʻitaʻiga fou ile 1 ml PBS, saline masani poʻo le buffer TE. Faʻamaeʻa uma homogenize le faʻataʻitaʻiga e se homogenizer ma ao le oso i lalo o le paipa e centrifuging. Tuʻu ese le supernatant, ma resuspend le oso i le 200 μl buffer GA. Ole faʻamamaina ole DNA e mafai ona faia e tusa ma faʻatonuga.
Mo le faʻamamaina o gDNA i plasma, serum ma tino faʻataʻitaʻiga suavai, TIANamp Micro DNA Pusa ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum / faʻataʻitaʻiga plasma, TIANamp Virus DNA / RNA Kit ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum ma faʻataʻitaʻiga plasma, TIANamp Bacteria DNA Pusa ua fautuaina (lysozyme tatau ona aofia ai mo lelei siama). Mo faʻataʻitaʻiga faua, Hi-Swab DNA Pusa ma TIANamp Bacteria DNA Pusa ua fautuaina.
DNAsecure Plant Pusa poʻo DNAquick Plant System e fautuaina mo le aveʻese mai o le genome. Mo le toʻafilemuina o le genome, TIANamp Yeast DNA Kit e fautuaina (lyticase tatau ona saunia e ia lava).
Vaega o oloa
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