■ O faʻailoilo lanu e faʻaopopoina i mea moni e aloese mai faʻataʻitaʻiga faʻaopoopo sese pe le faia ona o le tele numera o faʻataʻitaʻiga.
■ Maualuga maualuga fluorescence taua, maualuga maaleale ma maualuga faʻapitoa.
■ A maeʻa ona maitauina saʻo, o le faʻailoga o le a le afaina ai le aoga o le fluorescence ma le aofaʻi o iʻuga o le vali.
■ E fetaui lelei mo ituaiga eseese o mea masani ole PCR masani (Roche, Bio-rad, ABI, ma isi).
E fetaui lelei mo ituaiga eseese o faʻataʻitaʻiga e pei o faʻamatalaga auiliiliina ma le faʻamaoniga o le acidic nucleic e le SYBR Green metotia i luga o le tele o taimi moni mea faigaluega PCR. E sili ona talafeagai mo maualuga alualu i luma faʻataʻitaʻiga ma le tele numera o faʻataʻitaʻiga ma faigata faʻataʻitaʻiga utaina faʻagaioiga.
Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)
Faʻatusa 1. Faʻailoga o faʻasologa fua faʻalautelega ma liusuavai liʻua na maua e ala ile fuaina o le tagata HS1 gene e faʻaaoga ai TIANGEN SuperReal PreMix Plus (SYBR Green) (A) ma le SuperReal PreMix Colour (SYBR Green) (B). O le faʻataʻitaʻiga ua faʻavaivaia mo le 5 faʻapitoa gradients. O le Ct taua (C) maua i faʻatusatusaga faʻaalia ai o le faʻateleina o le lua pusa i ai tele malamalama, faʻapitoa ma mautu. O le faʻataʻitaʻiga na tamoe i luga Roche LightCycler480. | |
Ata 2. Faʻatusa o le faʻateleina o le piʻoga ma le faʻaliʻaliʻuga faʻatumuina maua mai i le faʻatulagaina o le aofaʻi o le tagata HS5 gene e faʻaaoga ai TIANGEN SuperReal PreMix Colour (SYBR Green) ma oloa talafeagai mai le Supplier T (A: TIANGEN, B: Supplier T. Faʻafuaseʻi le faʻataʻitaʻiga mo le 5 faʻasologa lelei gradients, ma faʻatusatusa le taua Ct (C) O le iʻuga ua faʻaalia ai o le SuperReal PreMix Colour (SYBR Green) e maualuga lona malamalama, faʻapitoa ma mautu. |
Talu mai lona faavaeina, o lo tatou fale gaosi ua atiina ae muamua lalolagi vasega oloa ma mulimulitaia le mataupu faavae
o lelei muamua. Tatou oloa ua maua sili lelei taʻuleleia i le alamanuia ma valuabletrusty i fou ma tuai tagata faʻatau ..