RNAprep Pure Hi-Blood Pusa

Mo le faʻamamaina o maualuga-lelei ma mautu RNA aofaʻi mai le toto.

O le RNAprep Pure Hi-Blood Kit e sili ona lelei ona aveʻesea uma le RNA mai le toto atoa ma le toto ma le tele o vailaʻau oona mai ituaiga eseese. O le silicon matrix mea na faʻaaogaina i le adsorption koluma o se tulaga ese fou mea na atiaʻe e TIANGEN, e lelei ma faʻapitoa faʻapitoa faʻasalalau RNA, ma aveʻesea polotini polotini i le mea silisili. E mafai ona faʻaaogaina le RNA na maua mai i faʻataʻitaʻiga iloga e pei ole RT-PCR, RT-qPCR, chip analysis, high-throughput sequencing, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molekule cloning, etc.

Pusi leai	Tele teuina
4992903	50 preps

Lafo imeli ia matou

Oloa Faʻamatalaga

Faʻataʻitaʻiga Faʻataʻitaʻiga

FAQ

Pine o oloa

Features

■ E fetaui lelei mo le toto fou o ituaiga eseese, e faigofie ona faʻagaioia.
■ Faʻaauupegaina i le RNase-Free Filtration Column CS e aveʻese lelei ai le le mama.
■ O le faʻapitoa faʻapitoa buffer mafai faʻamautinoa lelei lelei ma mautu RNA toʻesea mo le tele o ituaiga i lalo faʻataʻitaʻiga.
■ Faʻagaioiga saogalemu ma le faʻatuatuaina, e leai se phenol / chloroform aveʻese mai manaʻomia.

Talosaga

RT-PCR, North Blot, RT-qPCR, chip analysis, high-throughput sequencing, PolyA screening, RNase protection analysis, in vitro translation, etc.

Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)

Talu ai: RNAprep Laʻau Laʻau Laʻau Maea Lelei

Sosoo ai: RNA Faigofie Vave Tissue / Pusa sela

	Ata 1. RNA faʻamamaina mai 100 μl fou isumu toto i vailaʻau anticoagulants faʻaaogaina RNAprep Pure Hi-Blood Kit. 4-6 μl o 50 μl eluates na utaina ile laina. M: TIANGEN DNA Faʻailoga III.
	Ata 2. RNA faʻamamaina mai le 100 μl toto isumu fou faʻaaogaina RNAprep Pure Hi-Blood Kit. 4-6 μl o le 50 μl eluates na utaina ile laina. M: TIANGEN DNA Faʻailoga III.

F: Kolisi poloka

A-1 Cell lysis poʻo le homogenization le lava

---- Faʻaititia faʻataʻitaʻiga faʻaaogaina, faʻateleina le aofaʻi o lysis buffer, faʻateleina homogenization ma lysis taimi.

A-2 Sample aofaiga e telē tele

---- Faʻaititia le aofaʻi o faʻataʻitaʻiga faʻaaogaina pe faʻateleina le aofaʻi o lysis buffer.

Q: Fua maualalo RNA fua

A-1 Le lava le sela lysis poʻo le homogenization

---- Faʻaititia faʻataʻitaʻiga faʻaaogaina, faʻateleina le aofaʻi o lysis buffer, faʻateleina homogenization ma lysis taimi.

A-2 Sample aofaiga e telē tele

---- Faʻamolemole vaʻai i le maualuga gafatia gafatia.

A-3 RNA e le elied atoa mai le koluma

---- A maeʻa ona faʻaopopo le RNase-Free vai, tuʻu mo ni nai minute ae le i totogia le centrifuging.

A-4 Ethanol i le eluent

---- A maeʻa le mulumulu, toe faʻatulaga lelei ma aveese le faʻamago i le mea sili e mafai.

A-5 Cell aganuu auala e le maeʻa uma

---- Pe a faʻaputuputuina sela, faʻamolemole ia mautinoa ia aveʻese le aganuʻu auala i le tele e mafai ai.

A-6 O sela o loʻo teuina ile RNAstore e leʻo totogia lelei

---- RNAstore density e sili atu nai lo le averesi sela aganuu auala; o lea e tatau ona faʻateleina le centrifugal force. E fautuaina e centrifuge ile 3000x g.

A-7 Low RNA mea ma tele i le faʻataʻitaʻiga

---- Faʻaaoga se faʻataʻitaʻiga lelei e faʻamautinoa ai pe o le maualalo o fua e mafua mai i le faʻataʻitaʻiga.

Q: RNA faʻaleagaina

A-1 O mea e le fou

---- O mea fou e tatau ona teuina i totonu o le vai nitrogen i le taimi lava lena poʻo le vave tuʻuina i totonu o le RNAstore reagent e mautinoa ai le aveʻeseina aʻafiaga.

A-2 Sample aofaiga e telē tele

---- Faʻaititia le aofaʻi aofaʻi.

A-3 RNase faʻaleagainan

---- E ui lava e leʻo iai le RNase i le buffer o loʻo i totonu o le pusa, e faigofie lava ona faʻaleagaina le RNase i le taimi e aveese ai ma e tatau ona faʻatautaia ma le faʻaeteete.

A-4 Faʻaleagaina eletise

---- Sui le paipa eletise ma ia mautinoa o mea faʻaaoga ma Uta Faʻafefe e leai se faʻamaʻi RNase.

A-5 Tele tele utaina mo electrophoresis

---- Faʻaitiitia le aofaʻi o faʻataʻitaʻiga utaina, o le utaina o vaiʻeli uma e le tatau ona sili atu i le 2 μg.

Q: DNA faʻaleagaina

A-1 Faʻataʻitaʻiga aofaʻi e telē tele

---- Faʻaititia le aofaʻi aofaʻi.

A-2 Nisi faʻataʻitaʻiga e maualuga aano o DNA ma e mafai ona togafitia ile DNase.

---- Faia togafitiga RNase-Free DNase ile tali RNA na maua, ma e mafai ona faʻaaoga saʻo le RNA mo suʻesuʻega mulimuli ane pe a maeʻa togafitiga, pe mafai foi ona faʻamamaina e RNA faʻamamaina ato.

Q: Faʻafefea ona aveese le RNase mai faʻataʻitaʻiga faʻaaoga ma tioata?

Mo tioata, tao i le 150 ° C mo le 4 h. Mo palasitika koneteina, faʻatofu i le 0.5 M NaOH mo le 10 min, ona fufulu faʻamaeʻa lea i le RNase-leai se vai ona faʻamamaina lea e aveʻese atoa le RNase. O le reagents poʻo vaifofo faʻaogaina i le faʻataʻitaʻiga, aemaise lava le vai, tatau ona saoloto mai RNase. Faʻaaoga le vai e leai se RNase mo sauniuniga uma (faʻaopopo vai i se fagu tioata mama, faʻaopopo le DEPC i le faʻaiʻuga mulimuli o le 0.1% (V / V), lulu le po ma le autoclave).

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