RNA Faigofie Vave Laʻau Tisini Pusa
Features
■ Faigofie ma vave: Aofaʻiga RNA toʻesea mai laʻau faʻataʻitaʻi mafai ona maeʻa i totonu o le 30 minute.
■ Malosi faʻamalosia: O lenei pusa e le gata e talafeagai mo masani ai laʻau ma laulaʻau faʻataʻitaʻi, ae faʻapea foi mo polysaccharide / polyphenol-rich sample.
■ Saogalēmū ma maualalo le 'oʻona: O vailaʻau oona e pei o le β-mercaptoethanol, DTT, chloroform, phenol e le manaʻomia.
Talosaga
■ E talafeagai mo le faʻagaio i lalo, e aofia ai le RT-PCR, RT-qPCR, puʻeina hybridization, Northern Blot, Dot Blot, PolyA faʻataʻitaʻiga, i vitro faʻaliliuga, RNase puipuiga faʻamalamalamaga ma molikalone faʻapipiʻi, ma isi.
Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)
 |
O le aofaʻi o le RNA o le 65 mg laʻau faʻataʻitaʻi (laʻau afu, laʻau hibiscus, lau saito, lau araisa, ugli fualaʻau lau, Prunus cerasifera lau, lau lau, daylily lau, Kerria japonica lau), 150 mg faʻataʻitaʻiga fungus (Lentinus edodes) ma 200 mg petal sampels (day-lily petals) na aumaia e faʻaaoga ai le RNA Easy Fast Plant Tissue Kit. Agilent Bioanalyzer 2100 faʻaiʻuga o le aofaʻi o RNA mai Day-lily petals. |
 |
Agilent Bioanalyzer 2100 auiliiliga faʻaiuga o le aofaʻi o le RNA mai le Day-lily petals. |
 |
Na maua mai le RNA mai faʻataʻitaʻiga eseese ua lisiina i le laulau e faʻaaoga ai le RNA Easy Fast Plant Tissue Kit. Voluma Elution: 50 μl; Utaina voluma: 2 μl O le electrophoresis na faia i le 6 V / cm mo le 15 min i le 1% agarose. |
A-1 Cell lysis poʻo le homogenization le lava
---- Faʻaititia faʻataʻitaʻiga faʻaaogaina, faʻateleina le aofaʻi o lysis buffer, faʻateleina homogenization ma lysis taimi.
A-2 Sample aofaiga e telē tele
---- Faʻaititia le aofaʻi o faʻataʻitaʻiga faʻaaogaina pe faʻateleina le aofaʻi o lysis buffer.
A-1 Le lava le sela lysis poʻo le homogenization
---- Faʻaititia faʻataʻitaʻiga faʻaaogaina, faʻateleina le aofaʻi o lysis buffer, faʻateleina homogenization ma lysis taimi.
A-2 Sample aofaiga e telē tele
---- Faʻamolemole vaʻai i le maualuga gafatia gafatia.
A-3 RNA e le elied atoa mai le koluma
---- A maeʻa ona faʻaopopo le RNase-Free vai, tuʻu mo ni nai minute ae le i totogia le centrifuging.
A-4 Ethanol i le eluent
---- A maeʻa le mulumulu, toe faʻatulaga lelei ma aveese le faʻamago i le mea sili e mafai.
A-5 Cell aganuu auala e le maeʻa uma
---- Pe a faʻaputuputuina sela, faʻamolemole ia mautinoa ia aveʻese le aganuʻu auala i le tele e mafai ai.
A-6 O sela o loʻo teuina ile RNAstore e leʻo totogia lelei
---- RNAstore density e sili atu nai lo le averesi sela aganuu auala; o lea e tatau ona faʻateleina le centrifugal force. E fautuaina e centrifuge ile 3000x g.
A-7 Low RNA mea ma tele i le faʻataʻitaʻiga
---- Faʻaaoga se faʻataʻitaʻiga lelei e faʻamautinoa ai pe o le maualalo o fua e mafua mai i le faʻataʻitaʻiga.
A-1 O mea e le fou
---- O mea fou e tatau ona teuina i totonu o le vai nitrogen i le taimi lava lena poʻo le vave tuʻuina i totonu o le RNAstore reagent e mautinoa ai le aveʻeseina aʻafiaga.
A-2 Sample aofaiga e telē tele
---- Faʻaititia le aofaʻi aofaʻi.
A-3 RNase faʻaleagainan
---- E ui lava e leʻo iai le RNase i le buffer o loʻo i totonu o le pusa, e faigofie lava ona faʻaleagaina le RNase i le taimi e aveese ai ma e tatau ona faʻatautaia ma le faʻaeteete.
A-4 Faʻaleagaina eletise
---- Sui le paipa eletise ma ia mautinoa o mea faʻaaoga ma Uta Faʻafefe e leai se faʻamaʻi RNase.
A-5 Tele tele utaina mo electrophoresis
---- Faʻaitiitia le aofaʻi o faʻataʻitaʻiga utaina, o le utaina o vaiʻeli uma e le tatau ona sili atu i le 2 μg.
A-1 Faʻataʻitaʻiga aofaʻi e telē tele
---- Faʻaititia le aofaʻi aofaʻi.
A-2 Nisi faʻataʻitaʻiga e maualuga aano o DNA ma e mafai ona togafitia ile DNase.
---- Faia togafitiga RNase-Free DNase ile tali RNA na maua, ma e mafai ona faʻaaoga saʻo le RNA mo suʻesuʻega mulimuli ane pe a maeʻa togafitiga, pe mafai foi ona faʻamamaina e RNA faʻamamaina ato.
Mo tioata, tao i le 150 ° C mo le 4 h. Mo palasitika koneteina, faʻatofu i le 0.5 M NaOH mo le 10 min, ona fufulu faʻamaeʻa lea i le RNase-leai se vai ona faʻamamaina lea e aveʻese atoa le RNase. O le reagents poʻo vaifofo faʻaogaina i le faʻataʻitaʻiga, aemaise lava le vai, tatau ona saoloto mai RNase. Faʻaaoga le vai e leai se RNase mo sauniuniga uma (faʻaopopo vai i se fagu tioata mama, faʻaopopo le DEPC i le faʻaiʻuga mulimuli o le 0.1% (V / V), lulu le po ma le autoclave).
Vaega o oloa
AISEA FILIFILI US
Talu mai lona faavaeina, o lo tatou fale gaosi ua atiina ae muamua lalolagi vasega oloa ma mulimulitaia le mataupu faavae
o lelei muamua. Tatou oloa ua maua sili lelei taʻuleleia i le alamanuia ma valuabletrusty i fou ma tuai tagata faʻatau ..
- Tel: +86 010-59822688
- Fale 5, Nu 86, Shuangying West Road, Changping District, Beijing.
- tagata@tiangen.com
