Maneta Manu Manu Tisini Genomic DNA Pusa

Tuʻua ma faʻamamaina o maualuga maualuga genomic DNA mai manu tino.

O le pusa vaetamaina manoa manoa ma tulaga ese faʻatinoina vavaeʻesega ma se tulaga ese buffer faiga e vavaeʻese ma faʻamama maualuga-lelei genomic DNA mai meaola meaola faʻataʻitaʻiga. E le masani ai ona faʻaaogaina manoa maneta i totonu o ni tulaga taua. Pe a suia tulaga, o le maneta lopa o le a faʻasaʻolotoina adsorbed nucleic acid, ma faʻapea ona mauaina le mafuaʻaga o le vave vavaeʻeseina ma le faʻamamaina o le nucleic acid. O le atoa gaioiga e saogalemu ma talafeagai. Faatasi ai ma lapoʻa aveʻesea genomic DNA fragments, maualuga mama ma faʻatuatuaina lelei, o le metotia e sili ona talafeagai mo otometi toʻesea mai o maualuga-throughput workstations.

Pusi leai	Tele teuina
4992404	50 preps
4992405	200 preps
4992864	1000 preps

Lafo imeli ia matou

Oloa Faʻamatalaga

Faʻataʻitaʻiga Faʻataʻitaʻiga

FAQ

Pine o oloa

Features

■ Faigofie ma vave: Direct lysis aunoa maʻi incubation, ma ultrapure genomic DNA mafai ona maua i totonu o le 1 itula.
■ Maualuga maualuga: E mafai ona tuʻufaʻatasia ma mea faigaluega otometi o le pipetting metotia ma maneta faʻavae metotia mo maualuga throughput aveʻesea o faʻataʻitaʻiga.
■ Saogalēmū ma lē oona: Leai ni meaola e pei o phenol / chloroform e manaʻomia.
■ Tulaga maualuga mama: O le DNA na mauaina e maualuga lona mama ma e mafai ona faʻaaoga saʻo i siama siaki, maualuga-throughput faʻasologa ma isi faʻataʻitaʻiga.

Talosaga

O le faʻamamaina DNA mafai ona faʻaogaina i le tele o faʻataʻitaʻiga faʻataʻitaʻiga e pei o le enzyme digestion, PCR, faletusi fausiaina, South hybridization, chip hybridization, maualuga-throughput sequencing, ma isi.

Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)

Talu ai: Serum / Plasma Faʻasalalauina DNA Pusa

Sosoo ai: Maneta maneta toto Toto DNA Pusa

Faʻaaoga le Magnetic Animal Tissue Genomic DNA Kit e faʻamama ai le DNA mai tino eseese o isumu, iʻa, ula, atigi, sela faʻatupu, pato vae povi ma isi faʻataʻitaʻiga e tusa ai ma faʻatonuga. M: TIANGEN Faʻailoga D15000.
Eluma voluma e 150 µl. 1-2 µl eluent na utaina i luga ole agarose gel electrophoresis.
Iʻuga: TIAGNEN Magnetic Animal Tissue Genomic DNA Kit o loʻo iai le tele o faʻataʻitaʻiga e mafai ona faʻaaogaina, aemaise lava mo faʻataʻitaʻiga faigata e pei o isumu isumu, iʻa, ula ma figota.

Faʻaaoga le TIAGNEN Magnetic Animal Tissue Genomic DNA Kit ma pusa talafeagai o le Supplier M, O, A e faʻamama le DNA mai le 25 mg rat heart tissue e tusa ai ma faʻatonuga. M: TIANGEN Faʻailoga D15000.
Voluma Elution: 150 µl. 1 µl eluent na utaina i luga ole agarose gel electrophoresis.
Iʻuga: TIAGNEN Magnetic Animal Tissue Genomic DNA Kit e maualuga atu lona mauaina mai.

Q: Laʻititi pe leai foi DNA i le eluent.

A-1 Maualalo le totoina o sela poʻo siama i le amataga o le faʻataʻitaʻiga - Faʻamalosia le faʻasologa o sela poʻo siama.

A-2 Lysis le lava o faʻataʻitaʻiga - O faʻataʻitaʻiga e leʻi maeʻa maeʻa faʻafefiloi ma le buffer lysis. E fautuaina e fefiloi maeʻa ile pulse-vortexing mo le 1-2 taimi. —Ua le lava le sela o le sela na mafua mai i le faʻaititia o proteinase K. - Le lava le lysis sela poʻo le faʻaleagaina o le polotini ona o le le lava o le vai tavela mafanafana. E fautuaina e tipi le fasi pepa i ni fasi laiti ma faʻalautele le taimi taele e aveʻese uma toega i le lysate.

A-3 Le lava faʻasalalauga DNA. —E leai se ethanol poʻo se maualalo pasene nai lo le 100% ethanol na faʻaopopoina ae le i faʻamatuʻu le lysate i le vilivalavala.

A-4 O le taua ole pH ole faʻaola eletise e maualalo tele. —Faiaso le pH i le va o le 8.0-8.3.

Q: E leʻo lelei le faʻagaioiga ole DNA ile faʻataʻitaʻiga ole faʻasolosolo ole enzymatic.

Ethanol totoe i le eluent.

—E i ai toega fufuluina PW i le eluent. O le ethanol e mafai ona aveʻese i le centrifuging le vili pou mo 3-5 min, ona tuʻu ai lea i le potu vevela poʻo le 50 ℃ incubator mo 1-2 min.

Q: DNA faʻaleagaina

A-1 O le faʻataʻitaʻiga e le fou. — Aveʻese se faʻataʻitaʻiga lelei o le DNA e fai ma faʻatonutonu e faʻamautinoa ai pe ua faʻaleagaina le DNA i totonu o le faʻataʻitaʻiga.

A-2 Leaga muamua togafitiga. —Faʻailoaina e le soʻona suavaia oona nitrogen, toe maua le susu, poʻo tele tele o le faʻataʻitaʻiga.

Q: Faʻafefea ona faʻatinoina le muaʻi faʻaaliga mo le aveʻeseina o le gDNA?

E tatau ona fesuisuiaʻi mea e fai mo faʻataʻitaʻiga eseese. Mo faʻataʻitaʻiga laʻau, ia mautinoa ia olo lelei i le nitrogen suavaia. Mo manu faʻataʻitaʻiga, homogenate poʻo olo lelei i le suavaia oona. Mo faʻataʻitaʻiga ma sela sela e faigata ona gagau, pei o le G + bacteria ma le fefete, e fautuaina e faʻaoga le lysozyme, lyticase poʻo metotia e motusia ai sela o sela.

Q: O le a le eseesega i le va o le tolu laau gDNA aveʻesea pusa 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Laʻau Genomic DNA Kit faʻaaogaina se koluma-faʻavae metotia e manaʻomia chloroform mo le aveʻesea. E sili ona talafeagai mo eseese fualaʻau faʻataʻitaʻi, faʻapea foi ma laʻau mago paʻu. O le Hi-DNAsecure Plant Kit o loʻo faʻavaeina foi i luga ole koluma, ae leai se manaʻoga o le faʻateʻaina o le phenol / chloroform, e saogalemu ai ma e le oona foi. E fetaui lelei mo laʻau ma maualuga polysaccharides ma polyphenol mea. 4992709/4992710 DNAquick Plant System faʻaogaina se vai-faʻavae metotia. Phenol / chloroform aveʻesea e le manaʻomia foi. O le faʻamamaina gaioiga e faigofie ma vave ma leai se tapulaʻa mo le amataga amataga aofaʻiga, o lea e mafai ai e tagata faʻaoga ona fetuʻunaʻi le aofaʻi fetuʻunaʻi e tusa ai ma faʻataʻitaʻiga manaʻoga. Tele lapoa o gDNA fasi e mafai ona maua ma maualuga fua.

O le a le faʻatatauina maua o gDNA mai le 1 ml toto faʻataʻitaʻiga e TIANamp Toto DNA Pusa?

O le genomic DNA na aumaia mai vaega eseese o faʻataʻitaʻiga o le toto o le tagata e le TIANamp Blood DNA Kit. O iʻuga e mulimuli mai. O iʻuga e lisiina naʻo faʻasino, o le mea moni toʻeseʻesea iʻuga e faʻamoemoeina i tuutuuga o faʻataʻitaʻiga.

F: Mafai e le 4992207/4992208 ma le 4992722/4992723 ona faʻaaogaina e aveʻesea ai le toto masaa o le DNA?

E mafai ona faʻatino le vailaʻau toto DNA e faʻaaoga ai meaʻai na saunia i nei mea e lua i le naʻo le suia o le maliega faʻapitoa i faʻatonuga faʻapitoa mo le aveʻesea o le toto o le DNA. O le kopi vaivai o le toto clot DNA aveʻesega maliega maliega faʻapitoa mafai ona tuuina atu pe a talosagaina.

F: Pe a faʻaogaina le TIANamp Genomic DNA Kit, faʻafefea ona gagau ia mea fou i totonu o le sela taofia?

Taofi le faʻataʻitaʻiga fou ile 1 ml PBS, saline masani poʻo le buffer TE. Faʻamaeʻa uma homogenize le faʻataʻitaʻiga e se homogenizer ma ao le oso i lalo o le paipa e centrifuging. Tuʻu ese le supernatant, ma resuspend le oso i le 200 μl buffer GA. Ole faʻamamaina ole DNA e mafai ona faia e tusa ma faʻatonuga.

Q: Faʻafefea ona filifili le oloa mo le DNA toʻesea mai plasma, serum ma tino faʻataʻitaʻiga suavai?

Mo le faʻamamaina o gDNA i plasma, serum ma tino faʻataʻitaʻiga suavai, TIANamp Micro DNA Pusa ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum / faʻataʻitaʻiga plasma, TIANamp Virus DNA / RNA Kit ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum ma faʻataʻitaʻiga plasma, TIANamp Bacteria DNA Pusa ua fautuaina (lysozyme tatau ona aofia ai mo lelei siama). Mo faʻataʻitaʻiga faua, Hi-Swab DNA Pusa ma TIANamp Bacteria DNA Pusa ua fautuaina.

Q: Faʻafefea ona filifili ato mo le aveʻesea o le gDNA mai faʻataʻitaʻiga mai le fungi?

DNAsecure Plant Pusa poʻo DNAquick Plant System e fautuaina mo le aveʻese mai o le genome. Mo le toʻafilemuina o le genome, TIANamp Yeast DNA Kit e fautuaina (lyticase tatau ona saunia e ia lava).

Pusa E leai se faʻaaogaina Dna Aveʻiga Pusa