■ Sili ona vave: Antibody fesuiaʻi Aneti Taq DNA polymerase, galulue faʻatasi ma le tulaga ese vave Buffer faiga, sefe e oʻo i le 60% o taimi tali ma avea ma sili ona vave SYBR Green soʻotaga i luga o le maketi i le taimi nei.
■ Malosiaga faʻateleina agavaʻa: Malosi faʻateleina faʻailoilo fluorescence, sili saʻo ma talitonuina iʻuga.
■ Lelei mautu: O se tulaga ese PCR stabilizer ma enhancer ua faʻaopopoina i Buffer, faia le iʻuga sili mautu, toe fai, saʻo ma talitonuina.
■ lautele faʻaaogaina: E le gata talafeagai mo vave taimi moni PCR mea faifaaili, ae e talafeagai foi mo masani masani PCR mea faifaaili.
■ Faʻasaʻoga ROX: O le ROX vali e tuʻufaʻatasia afifi, e sili atu fetuʻutuʻunaʻi e faʻaoga ma mafai ona mautinoa ai le sili atu saʻo o iʻuga.
Ituaiga: Antibody-fesuiaʻi vevela-amata DNA polymerase, SYBR Green I
Taimi o faʻagaioiga: ~ 30 min
Talosaga: Dye-based qPCR mo faʻamaoniga o kenera i luga o le DNA poʻo faʻamatalaga faʻapitoa i luga o faʻataʻitaʻiga cDNA mai ituaiga eseese
Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)
Malosiaga faʻateleina faʻalauteleina Vave-Faʻaleleia le faʻataʻitaʻiga lelei, faʻafualoa le tautua ola o le mea faigaluega ma sefe le malosiʻaga Taq DNA polymerase mafai ona faʻagaoioi vave, faʻamautinoaina FastFire oloa avea ma sili ona vave SYBR Green faʻamalosia luga o le maketi i le taimi nei ma se tulaga ese vave Buffer faiga e faʻaleleia atili ai le lelei faʻataʻitaʻiga. |
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Malosiaga faʻateleina faʻalauteleina O le faʻalauteleina fluorescence faailo e sili atu malosi (amplification mafai gafatia e malosi), ma le sili atu tulaga faʻateleina piʻoga, maualuga maʻaʻa, ma e mafai ma le saʻo ma le tele faʻatauaina le sini autu o le maualalo-faʻasologa template. O le faʻailoiloina o faʻamatalaga talafeagai mai le Supplier T e vaivai, ma o le CT taua e i tua, lea e ono taitai atu ai i le saʻo o iʻuga. |
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96-lelei tutusa faʻataʻitaʻiga faʻataʻitaʻi faʻaalia ai o le reagent e sili atu le mautu ma sili atu faʻafouga. |
Talu mai lona faavaeina, o lo tatou fale gaosi ua atiina ae muamua lalolagi vasega oloa ma mulimulitaia le mataupu faavae
o lelei muamua. Tatou oloa ua maua sili lelei taʻuleleia i le alamanuia ma valuabletrusty i fou ma tuai tagata faʻatau ..