EndoFree Maxi Plasmid Pusa V2

Faʻamamaina o endotoxin-leai se suiga vasega plasmid DNA faʻapitoa mo sela maaleale.

O le EndoFree Maxi Plasmid Kit V2 o loʻo faʻaaogaina se auala tulaga ese o le silica membrane adsorption i se auala lelei ma faʻapitoa e fusia ai le plasmid DNA. O le faʻapitoa Buffer ER ma le Filtration CS1 mafai mafai ona aveʻesea lelei impurities pei o endotoxin ma porotini. O le faʻagasologa atoa o le aveʻesega e naʻo le 1 itula le umi.
Fautuaina siama aganuu feololo feololo aofaʻi: Mo maualuga-kopi plasmid, 100 ml siama auala e fautuaina, lea e mafai ona maua ai oʻo atu i le 500-1500 μg plasmid. Mo maualalo-kopi plasmid, 200 ml o siama auala e fautuaina e ausia le fua o le lata i le 50-300 μg.

Pusi leai Tele teuina
4992438 10 preps

Oloa Faʻamatalaga

Galue

Faʻataʻitaʻiga Faʻataʻitaʻiga

Pine o oloa

Features

■ Mama maualuga: O le tekonolosi tulaga ese endotoxin faʻatekonolosi ua faʻaaogaina e faʻapitoa aveʻesea endotoxin.
■ Faigofie ona faʻagaioia: O faʻasalalauga tekinolosi kolosi e faʻaaoga e faʻapitoa faʻasalalau ai plasmid DNA, e faʻafaigofie ai le faʻagaioiga.
■ Fesuiaʻiga sili ona aoga: Fetaui lelei mo le fesuiaʻiga o laina tele sela e aofia ai ma sela faʻaaogaina o le endotoxin.
■ Lautele ituaiga o talosaga: O le faʻamamaina plasmid mafai ona faʻaaogaina i le fesuiaʻiga o manu ma sela o sela ma faʻataʻitaʻiga o le biology.

Talosaga

O le plasmid DNA na aumaia e faʻaaoga ai lenei pusa e mafai ona faʻaaogaina mo gaioiga masani masani, e aofia ai le faʻatapulaʻaina o le enzyme digestion, PCR, faʻasologa, ligation, suiga ma le fesuiaʻiga o sela eseese.

Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)


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    Workflow

    Experimental Example 图片 2 O le eseʻesega endotoxin aveʻesea reagent Buffer ER mafai lelei aveʻese endotoxin toega i le tali atu faiga, ma maualuga-mamā plasmid mafai ona maua. O le toega o le endotoxin o le plasmid o le EU 0.1 EU / ml.
    Experimental Example O le plasmid faʻamamaina faʻaaogaina EndoFree Maxi Plasmid Pusa V2 ma tutusa oloa mai le Faʻatau 1 ma le Faʻatauina 2 o loʻo faʻaalia i le tutusa tutusa o elution buffer. 1 μl plasmid na utaina ile laina e faʻatatau ai le faʻatulagaina ole plasmid. 100 ng o le plasmid o lona faʻatatauina na fuafuaina e le spectrophotometer na utaina i totonu o le tutusa gel e faʻamautinoa ai pe o le faʻatauaina taua na sese maualuga.
    Faaiuga: O le iʻuga ole electrophoresis o loʻo faʻaalia ai le faʻatulagaina moni o le plasmid na toʻesea mai e le EndoFree Maxi Plasmid kit V2, pe o le kopi maualuga poʻo le kopi laʻititi, e sili atu le maualuga nai lo le mea na aumai e le au faʻatau 1 ma le 2.
    Tusi lau feʻau ii ma lafo mai ia matou