TIANprep Rapid Mini Plasmid Kit Featured Image

TIANprep Rapid Iti Plasmid Pusa

Mo vave faʻamamaina o plasmid DNA o molemole biology vasega e alkaline lysis metotia.

O le TIANprep Rapid Mini Plasmid Kit ua faʻamautinoaina mai tekonolosi lysis masani alkaline, lea e mafai ai ona faʻamamaina DNA sili ona lelei DNA i totonu o le 8 minute. O le lysis buffer fou faʻatagaina le faʻailoaina o DNA i luga o le silica membrane ao iai le masima maualuga. O le mea na faia le silica membrane e faia e tulaga ese, sili ona lelei ma sili ona faʻapitoa. O lenei pusa ua fuafuaina mo le faʻamamaina e oʻo atu i le 24 μg plasmid DNA mai le 1-4 ml le tuʻufaʻatasi aganuu oE.coli. O le plasmid DNA faʻamamaina e lenei pusa e mafai ona faʻaaogaina i le tele o taimi masani faʻataʻitaʻiga, e pei o le enzyme digestion, PCR, faʻasologa, ligation, suiga, faletusi suʻesuʻega, in vitro transcription ma le fesuiaʻiga o masani sub-culture cells, ma isi.

Pusi leai	Tele teuina
4992191	50 preps
4992192	200 preps

Lafo imeli ia matou

Oloa Faʻamatalaga

Galue

Faʻataʻitaʻiga Faʻataʻitaʻiga

Pine o oloa

Features

■ Vave: Faigofie faʻagaioiga ma laʻititi laʻasaga e faʻamama maualuga maualuga plasmid DNA i totonu naʻo le 8 min.
■ Maualuga agavaʻa: Mafai ona faʻamamaina sili atu i le 85% plasmid DNA mai E.coli.
■ O le lanu faʻailoga TIANRed na sapalaiina e faʻatonutonu ma faʻamanatu ia gaioiga uma i le taimi o le faʻataʻitaʻiga e mautinoa ai le maualuga lelei o le faʻamamaina plasmid.

Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)

Talu ai: TGuide S32 Automated Nucleic Acid Extractor

Sosoo ai: EndoFree Midi Plasmid Pusa

Faatasi ai ma le maualuga-lelei lysate togafitiga ma optimized faiga buffer, TIANprep Rapid Mini Plasmid Pusa mafai ona faigofie maua sili maualuga lelei oloa ao sefe le tele o taimi faʻagaioiga.

Faʻatusatusaga i le va TIANprep Rapid Mini Plasmid Kit ma le Tagata Faʻatau A (lautele plasmid DNA faʻamamaina pusa) e ala i le faʻamamaina o le tutusa plasmid i le taimi e tasi.

Polokalame faʻataʻitaʻi: 3 ml tuʻufaʻatasi aganuu a E.coli (OD600 = 1.8); Voluma Elution: 50 μl; Utaina voluma: 1 μl pBS, 3 μl pET30a, 3 μl pBR322.

O le electrophoresis na faia i le 6 V / cm mo le 30 min i le 1% agarose gel. M: TIANGEN DNA Faʻailoga IV.

Faaiuga: Agarose gel electrophoresis faʻaalia o TIANprep Rapid Mini Plasmid Kit e mafai ona maua tutusa faʻatupuga o le plasmid DNA e pei o le lautele plasmid pusa faʻamamaina i totonu o le 8 min.

TIANprep Rapid Mini Plasmid Kit