■ Vave o le aveʻesega.
■ Faʻatumuina ma le tulaga ese TIANRed reagent, e mafai ona manino faʻailoaina le tikeri o le lysis o le faʻataʻitaʻiga i le tosoina mai o gaioiga ina ia mautinoa maualuga le lelei ma maualuga maualuga o le plasmid DNA.
■ Maualuga mama: O le mama plasmid DNA mafai ona faʻaaogaina saʻo i lalo ifo faataʻitaʻiga.
■ O lenei pusa e mafai ona faʻaaogaina i totonu o galuega tetele e faʻaaoga e faʻaaoga i lalo e faʻaaogaina ai le eletise.
Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)
Plasmid DNA na aumaia mai le 1 ml o le soʻoga galue Escherichia coli (TOP10) broth (LB auala) faʻaaogaina TIANprep Highpure N96 Plasmid Kit, ma le averesi o le 5-6 μg o maualuga maualuga mama pBS plasmid na maua. O le plasmid na utaina i vaieli taʻitasi o le 1% agarose gel, ma na maua e le electrophoresis |
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Plasmid DNA na aumaia mai le 1 ml o le soʻoga galue Escherichia coli (TOP10) broth (LB auala) faʻaaogaina TIANprep Highpure N96 Plasmid Kit, ma le averesi o le 5-6 μg o maualuga maualuga mama pBS plasmid na maua. Vector: pBS; Faʻamaʻi Plasmid: TOP10. O le plasmid na faʻasologa e le ABI. PRISM 3730XL DNA faʻasologa. |
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