TIANamp FFPE DNA Pusa
Features
■ Tulaga maualuga mama: O le silica membrane e faʻateʻaina le tele o mea leaga.
■ Lautele faʻaaogaina: Fetaui lelei mo paraffin vaega, paraffin poloka, ma formalin-faʻaaogaina faʻataʻitaʻiga.
■ Faagaioiga vave: DNA mafai ona maua i totonu o le 1 itula.
Faʻamatalaga
Ituaiga: Faʻavaʻa koluma faʻavae
Faʻataʻitaʻiga: Paraffin section, paraffin block, ma formalin-embedded faʻataʻitaʻiga
Manulauti: Genomic DNA
Amata le voluma: 5-8 vaega o paraffin vaega poʻo le 30 mg aano i paraffin poʻo formalin
Taimi o faʻagaioiga: ~ 1 itula
Talosaga i lalo ifo: PCR, SNP genotype analysis, STR auʻiliʻili, vailaʻau faʻamasaniina auiliiliga, NGS faletusi fausiaina, ma isi.
Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)
O le genomic DNA na aumaia e le TIANamp FFPE DNA Kit ma oloa talafeagai mai le Faʻatau A na faʻateleina i le 190 bp, 330 bp, 500 bp, 650 bp ma le 1300 bp fragments. O le iʻuga ua faʻaalia ai o le DNA na aumaia e le TIANamp FFPE DNA Kit e maualuga lona mauaina, maualuga le mama aemaise lava mo vaega tele.
Amataga mea: Isumu ate FFPE faʻataʻitaʻiga (5 fasi, 10 μm / fasi)
Taualumaga: Mulimuli i faʻatonuga o oloa taʻitasi.
Utaina o Gel: gDNA na faʻaalia e le 50 μl elution buffer ma na faʻateleina i le 190 bp, 330 bp 500 bp, 650 bp ma le 1300 bp. 5 μl o 20 μl PCR oloa na utaina ile laina ile 1% agarose gel. O le electrophoresis na faia i le 6 V / cm mo le 20 min.
M: TIANGEN Faʻailoga D2000;
T: TIANamp FFPE DNA Pusa;
A: Oloa talafeagai mai le Faʻatau A.
A-1 Maualalo le totoina o sela poʻo siama i le amataga o le faʻataʻitaʻiga - Faʻamalosia le faʻasologa o sela poʻo siama.
A-2 Lysis le lava o faʻataʻitaʻiga - O faʻataʻitaʻiga e leʻi maeʻa maeʻa faʻafefiloi ma le buffer lysis. E fautuaina e fefiloi maeʻa ile pulse-vortexing mo le 1-2 taimi. —Ua le lava le sela o le sela na mafua mai i le faʻaititia o proteinase K. - Le lava le lysis sela poʻo le faʻaleagaina o le polotini ona o le le lava o le vai tavela mafanafana. E fautuaina e tipi le fasi pepa i ni fasi laiti ma faʻalautele le taimi taele e aveʻese uma toega i le lysate.
A-3 Le lava faʻasalalauga DNA. —E leai se ethanol poʻo se maualalo pasene nai lo le 100% ethanol na faʻaopopoina ae le i faʻamatuʻu le lysate i le vilivalavala.
A-4 O le taua ole pH ole faʻaola eletise e maualalo tele. —Faiaso le pH i le va o le 8.0-8.3.
Ethanol totoe i le eluent.
—E i ai toega fufuluina PW i le eluent. O le ethanol e mafai ona aveʻese i le centrifuging le vili pou mo 3-5 min, ona tuʻu ai lea i le potu vevela poʻo le 50 ℃ incubator mo 1-2 min.
A-1 O le faʻataʻitaʻiga e le fou. — Aveʻese se faʻataʻitaʻiga lelei o le DNA e fai ma faʻatonutonu e faʻamautinoa ai pe ua faʻaleagaina le DNA i totonu o le faʻataʻitaʻiga.
A-2 Leaga muamua togafitiga. —Faʻailoaina e le soʻona suavaia oona nitrogen, toe maua le susu, poʻo tele tele o le faʻataʻitaʻiga.
E tatau ona fesuisuiaʻi mea e fai mo faʻataʻitaʻiga eseese. Mo faʻataʻitaʻiga laʻau, ia mautinoa ia olo lelei i le nitrogen suavaia. Mo manu faʻataʻitaʻiga, homogenate poʻo olo lelei i le suavaia oona. Mo faʻataʻitaʻiga ma sela sela e faigata ona gagau, pei o le G + bacteria ma le fefete, e fautuaina e faʻaoga le lysozyme, lyticase poʻo metotia e motusia ai sela o sela.
4992201/4992202 Laʻau Genomic DNA Kit faʻaaogaina se koluma-faʻavae metotia e manaʻomia chloroform mo le aveʻesea. E sili ona talafeagai mo eseese fualaʻau faʻataʻitaʻi, faʻapea foi ma laʻau mago paʻu. O le Hi-DNAsecure Plant Kit o loʻo faʻavaeina foi i luga ole koluma, ae leai se manaʻoga o le faʻateʻaina o le phenol / chloroform, e saogalemu ai ma e le oona foi. E fetaui lelei mo laʻau ma maualuga polysaccharides ma polyphenol mea. 4992709/4992710 DNAquick Plant System faʻaogaina se vai-faʻavae metotia. Phenol / chloroform aveʻesea e le manaʻomia foi. O le faʻamamaina gaioiga e faigofie ma vave ma leai se tapulaʻa mo le amataga amataga aofaʻiga, o lea e mafai ai e tagata faʻaoga ona fetuʻunaʻi le aofaʻi fetuʻunaʻi e tusa ai ma faʻataʻitaʻiga manaʻoga. Tele lapoa o gDNA fasi e mafai ona maua ma maualuga fua.
O le genomic DNA na aumaia mai vaega eseese o faʻataʻitaʻiga o le toto o le tagata e le TIANamp Blood DNA Kit. O iʻuga e mulimuli mai. O iʻuga e lisiina naʻo faʻasino, o le mea moni toʻeseʻesea iʻuga e faʻamoemoeina i tuutuuga o faʻataʻitaʻiga.
E mafai ona faʻatino le vailaʻau toto DNA e faʻaaoga ai meaʻai na saunia i nei mea e lua i le naʻo le suia o le maliega faʻapitoa i faʻatonuga faʻapitoa mo le aveʻesea o le toto o le DNA. O le kopi vaivai o le toto clot DNA aveʻesega maliega maliega faʻapitoa mafai ona tuuina atu pe a talosagaina.
Taofi le faʻataʻitaʻiga fou ile 1 ml PBS, saline masani poʻo le buffer TE. Faʻamaeʻa uma homogenize le faʻataʻitaʻiga e se homogenizer ma ao le oso i lalo o le paipa e centrifuging. Tuʻu ese le supernatant, ma resuspend le oso i le 200 μl buffer GA. Ole faʻamamaina ole DNA e mafai ona faia e tusa ma faʻatonuga.
Mo le faʻamamaina o gDNA i plasma, serum ma tino faʻataʻitaʻiga suavai, TIANamp Micro DNA Pusa ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum / faʻataʻitaʻiga plasma, TIANamp Virus DNA / RNA Kit ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum ma faʻataʻitaʻiga plasma, TIANamp Bacteria DNA Pusa ua fautuaina (lysozyme tatau ona aofia ai mo lelei siama). Mo faʻataʻitaʻiga faua, Hi-Swab DNA Pusa ma TIANamp Bacteria DNA Pusa ua fautuaina.
DNAsecure Plant Pusa poʻo DNAquick Plant System e fautuaina mo le aveʻese mai o le genome. Mo le toʻafilemuina o le genome, TIANamp Yeast DNA Kit e fautuaina (lyticase tatau ona saunia e ia lava).
Vaega o oloa
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