■ Faigofie e faʻaaoga: O le polokalame faʻagaioiga e mafai ona faigofie ona malamalama i totonu o le 1 h. O le fausia-i totonu PCR / qPCR sauniuniga polokalama mafai ona toe faʻaleleia ma lafo vave.
■ Faʻaogaina talafeagai: Fesoʻotaʻiga pipette pito ma Beckman Biomek 3000 polokalama.
■ Faigofie tausiga: O le otometi pipetting module (APM) mafai ona faigofie suia ma toe faafoi i tua mo debugging.
■ Maualuga maualuga ma le saʻo.
Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)
Ata 1: qPCR faʻataʻamilosaga faʻaiuga iʻuga faʻaalia lelei toe faʻaleleia cDNA faʻataʻitaʻiga o 7 μl NIH 3T3 sela ua faʻavaivaia 4 taimi ma le 21 μl o le vai i le fua faatatau o le 1: 4. Roche LightCycler 480 taimi moni fluorescence aofaʻi PCR mea faigaluega ma SuperReal PreMix Plus (SYBR Green) na faʻaaoga mo le mauaina. |
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Ata 2: Maualuga maualuga faʻatusatusa i le faʻasolosoloina o paipa (Tauagavale: Tusi Lesona; Taumatau: TEasy Automated Pipetting) 4 toe fai le faʻateleina ole tagata GAPDH (piʻo i luga). Faʻaopopo le 2 μl cDNA i le 18 μl MasterMix e fausia ai le 20 μl tali tali. Roche LightCycler 480 moni-taimi fluorescence aofaʻi PCR mea faigaluega ma SuperReal PreMix Plus (SYBR Green) na faʻaaoga mo le mauaina. |
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