Ia Tesema 2019, o le faʻasologa o niʻi niumonia o le le iloa mafuaʻaga na amata mai Wuhan, Hubei Itumalo, ma e leʻi umi ae sosolo i le tele o itumalo ma taulaga i Saina, ma le tele o isi atunuʻu ia Ianuari 2020. E oʻo atu i le 22:00 i le po Ianuari 27, 28 faʻamaoniaina mataupu ma 5794 masalomia 2019-nCov mataupu na lipotia i Saina. O lesiama mafuaʻaga o le siama na taʻua o Rhinolophus, ma o le taimi nei oti fua o 2.9%.
O Ianuari 12, 2020, na faʻalauiloa mai ai e le World Health Organization (WHO) o le faʻamaʻi pipisi na mafua mai i se ituaiga fou o le coronavirus (2019-nCov). Coronavirus o se ituaiga siama e sosolo i totonu o manu. O le siama nucleic acid ituaiga e tasi-paulia RNA. I le taimi lava e tasi, na faʻalauiloa ai foi e le WHO le faʻasologa o le faʻasologa o nicov o le nCov na faʻasoa e sikola Saina, lea na saunia ai le faʻavaeina o le molelaula mo le mauaina o siama ma ua mafai ai ona vave maua ma faʻailoaina faʻamaʻi.
Fai mai le faʻamatalaga a le WHO e faʻapea, o tagata e maua i faʻamaʻi manava manava e pei o le tale, fiva, faʻamaʻi o le respiratory tract ma sa oʻo atu i Wuhan i totonu o le 14 aso pe sa faʻafesoʻotaʻi ma isi tagata mamaʻi, e tatau ona maeʻa suʻesuʻeina. I le aso 17 Ianuari 2020, na faʻamalolo ai e le WHO le "Suʻesuʻega o le fale suʻesuʻe mo le 2019 novel coronavirus (2019-nCoV) i masalosaloga o tagata, Faʻatonuga le tumau, 17 Ianuari 2020". O le taʻitaʻiga faʻamanino atu o le specimens e aoina mai faʻailoga tagata maʻi aofia ai manava manava (nasopharynx ma oropharyngeal swabs, sputum, bronchoalveolar lavage, ma isi) ma serum faʻataʻitaʻiga, e pei ona taua i lalo:
* Mo le feaveaʻia o faʻataʻitaʻiga mo le mauaina o viral, faʻaaoga VTM (viral transport medium) o loʻo iai antifungal ma vailaʻau faʻafomaʻi. Mo siama pe faʻaleaganuʻu aganuu, felauaʻi mago pe i totonu o se laʻititi laititi o vai mama. Aloʻese mai le faʻaauau ona faʻaliliisa ma le liua o faʻataʻitaʻiga.
E ese mai i mea taua na aoina mai i luga o le laulau, ia mautinoa foi o loʻo avanoa isi masini ma mea faigaluega: eg container container ma specimen collection bag ma afifi, coolers ma cold packs poʻo le aisa mago, o totoga toto-toto meafaigaluega (eg nila, syringes ma tubes), igoa ma faʻailoga tumau, PPE, mea mo le faʻamamaina o luga.
Mo le mauaina o siama, e mafai ona faʻaaoga faʻataʻitaʻiga serum e faʻamaonia ai siama e ala i auala puipuia, ae o le RTqPCR e fautuaina e faʻailoa mai le nCov nucleic acid mo le saosaoa ma sili atu ona saʻo le mauaina. A o le siama
faʻasologa e iloa, o mea o le viral nucleic acid i le faʻataʻitaʻiga mafai ona vave mauaina e naʻo
filifilia filifilia talafeagai ma fetaui lelei muamua
(Suʻesuʻega faʻamaonia o Wuhan coronavirus 2019 e le taimi tonu RTPCR, Maliega ma faʻataʻitaʻiga muamua e pei o Ian 13, 2020) I le avea ai o se taʻitaʻi i le matata o le nucleic acid aveʻese ma mauaina i Saina, TIANGEN BIOTECH (BEIJING) CO., LTD. ua maua ai le siama-vae-gutu siama ma le fulū A (H1N1) siama mauaina vailaʻau mo sili atu i le 10 miliona tagata. I le 2019, o le siama o le TIANGEN's nucleic acid extraction ma le mauaina o vailaʻau ua uma ona faʻaoga i le 30 miliona Aferika masani puaa fiva faʻataʻitaʻiga, faia maoaʻe saofaga i le faʻamaoniga ma le puipuia o Aferika masani puaa fiva i Saina. O le TIANGEN e le gata o loʻo maua mai ai le saoasaoa ma le saʻo o le siʻitia o siama ma iloa ai mea moni, ae o loʻo maua ai foi ma talafeagai mea lelei ma mea faigaluega olo olo ma otometi vailaʻau oona o loʻo avea ma vaega atoa o fofo mo le aveʻesea o siama.
Automated Nucleic Acid Extraction Fofo
TIANGEN Automated Nucleic Acid Extractors e otometi lava faʻavae mo le faʻateʻaina o le acidic nucleic e faʻaaoga ai metotia maneta. O le faʻaaogaina o nei tulaga e le gata e faʻaititia ai le mamafa o galuega o asiasiga ma ofisa o le quarantine, ae faʻapena foi ona faʻaititia ai le faʻatinoina o mea sese o le aveʻesea o tusi lesona, ma mautinoa ai le tumau ma le mautu o le lelei o vailaʻau o loʻo maua mai iʻa.
TIANGEN Automated Nucleic Acid Extractor o loʻo i ai ni auala eseese (e aofia ai laina 16, 24, 32, 48, 96), ma e mafai ona faʻaaogaina ia tutusa mo le aveʻese mai o le acidic acid mai le tele o ituaiga faʻataʻitaʻiga. TIANGEN foi faʻaavanoaina tuʻufaʻatasia reagent atinaʻe ma mea faigaluega tuʻufaʻatasia tautua e tusa ai ma eseʻese manaʻoga manaʻoga.
TGrinder H24 Tisaini Homogenizer
● Lautele faʻaaoga mo olo ma homogenizing eseese aano o faʻamaʻi ma feces faʻataʻitaʻiga
● Faatasi ai ma le oloina homogenization malosiaga 2-5 taimi nai lo o mea masani tufugaaao
● olo lavalava ma homogenization, aloese mai le faʻaleagaina satauro
● Masini puipuia otometi e puipuia le saogalemu o tagata faigaluega falesuesue
TGuide S32 Automated Nucleic Acid Extractor
● Faʻataʻitaʻiga o galuega: 1-32 faʻataʻitaʻiga
● Voluma faʻagasolo: 20-1000 μl
● Ituaiga faʻataʻitaʻiga: Toto, sela, sela, palapala, siama ma isi faʻataʻitaʻiga
● Faʻagasologa o taimi: E oʻo atu ile 8 minute e maua mai ai le viral nucleic acid
● Faʻatonutonu auala: Faʻatonutonu faiga faʻatonutonu o Windows Pad ma le ki o le pupuni
● Faʻavae atinae: Tatala tulaga, saoloto e faʻafetaui reagents
● TGuide S32 Maneta Viral DNA / Pusa RNA (DP604)
● Lautele faʻaoga: E mafai ona faʻamamaina le maualuga o le siama DNA / RNA mai le serum, plasma, swab sample, faʻafofoina o vailaʻau ma fofo faʻamalosi siama.
● Faigofie ma lelei: O lenei oloa e fetaui lelei ma le TGuide S32 Automated Nucleic Acid Extractor, e mafai ona maua mai ai siama DNA / RNA ma maualuga fua, maualuga mama, mautu ma faʻatuatuaina uiga lelei.
● Talosaga i lalo: O le faʻamamaina o le nucleic acid e talafeagai mo faataʻitaʻiga i lalo o siama PCR ma taimi moni PCR.
Tusi lesona Nucleic Acid Extraction Solution
I le avea ai o se taʻimua i le vaega o le nucleic acid aveʻese ma mauaina, TIANGEN o loʻo ia te ia le sili ona auiliili o faʻasologa o meaola natiʻese mai oloa i Saina, e talafeagai lelei mo coronavirus mauaina mai ituaiga uma o faʻataʻitaʻiga: toto, serum / plasma, tino, swab, siama , ma isi
TIANamp Virus DNA / RNA Pusa (DP315)
● Maualuga lelei: Maualuga lelei siama DNA ma RNA mafai ona maua e ala i le faʻamamaina vave ma maualuga toe mafai.
● Maualuga mama: Atoa le aveʻesea o mea filogia ma mea e faʻapipiʻiina mo le faʻaaogaina i lalo.
● Maualuga le saogalemu: E le manaʻomia le tosoina mai o vailaʻau poʻo le etanol.
RNA Faʻamaʻi siama siama
1. TEasy Automated Pipetting System
● Maualuga maualuga: O le poloka malulu mafai ona tausia le vevela o le reagent faʻataʻitaʻiga lalo 7 ℃ mo sili atu i le 60 min. O APM taʻitasi e faʻatulagaina e tusa ma tulaga faʻavaomalo, lea e maualuga lona maualuga nai lo le faʻataʻinaina o laina paipa.
● Faigofie faʻagaioiga: Laʻititi tele. Mamafa mamafa. Leai se mea faigaluega e manaʻomia mo poloka sui sui. Fausiaina i totonu PCR / qPCR sauniuniga taualumaga. Faigofie tusi lesona faʻagaioiga o PCR fofo sauniuniga.
● Lautele tusi talosaga: Mafai ona faʻaaogaina mo le 96/384-faʻapipiʻi ipu paipa, PCR, qPCR, faʻailogaina o gafa ma isi faʻataʻitaʻiga maualuga.
FastKing One Step RT-qPCR Pusa (Faʻataʻitaʻiga) (FP314)
O le FastKing One Step RT-qPCR Kit (Probe) (FP314) na atiaʻe e TIANGEN o se onestep fesuiaʻi faʻaliliuina fluorescence aofaʻi aofaʻi mauaina pusa faʻavae i luga o metotia suʻesuʻega, lea ua fuafuaina faapitoa mo le mauaina o faʻailoga kenera i tele faʻataʻitaʻiga. O le KingRTase i totonu o le pusa o se mole mole fesuiaʻiga faʻafouina transcriptase, lea e sili atu le malosi RNA vavalalata ma vevela mautu, ma le faʻaleleia o le toe faʻafouina transcript lelei ma le faʻaopopoina agavaʻa o laʻasaga tulaga lua laʻasaga RNA faʻataʻitaʻiga. O se fou vevela amata Taq DNA polymerase o loʻo faʻaogaina foi e tuʻuina atu ai i le PCR tali maualuga le faʻamalosia lelei ma le faʻapitoa. I se faʻaopopoga, o le pusa e faʻafaigofieina vaega i le mea sili ona tele e ala i le palu faʻatasi o le Taq ma le MLV enzyme i totonu o le enzyme mix, ma le muaʻi paluina o le ion buffer, dNTPs, PCR stabilizer ma faʻalauteleina i le MasterMix, ina ia mafai ai ona faʻafesoʻotaʻi le tele o vaega.
faafaigofieina
● Maualuga lelei: O le sili atu fesuiaʻi transcriptase ma DNA polymerase mautinoa maualuga tali lelei gaioiga
● Lelei toe fesuiaʻiga: O le polymerase mafai faitau i totonu o le RNA faʻataʻitaʻiga ma maualuga GC mataupu ma faigata lua faʻusaga
● Lautele tusi talosaga: Maualuga faʻaaogaina i RNA faʻataʻitaʻiga ma le le mama mai ituaiga eseese
● Maualuga le agavaʻa: O faʻataʻitaʻiga maualalo pei o le 1 ng e mafai ona faʻailoa saʻo, ae maise lava mo le maualalo o faʻataʻitaʻiga
Faʻataʻitaʻiga mo le RNA Virus Detection
O le nucleic acid o le H5 avian influenza na aumaia e le TGuide S32 Magnetic Viral DNA / RNA Kit (DP604). O le FastKing One Step RT-qPCR Kit (Probe) (FP314) na faʻaaoga mo le mauaina o le RT-qPCR i le faʻaaogaina o le H5 avian influenza primers ma probes.
ABi7500Fast na faʻaaogaina mo le mauaina o le RT-qPCR. O iʻuga o le H5 avian influenza virus antigen (10-2, 10-3, 10-4, 10-5, 10-6and 10-7 dilution) mai le 200 μl faʻataʻitaʻiga faʻaalia ai le maualuga siama faʻamaʻi ninii nucleic acid maua mai, lea e mafai ona faʻamalieina le mulimuli ane manaʻoga o fesuiaʻiga tusitusi, PCR, RT-PCR, taimi moni PCR, ma isi O le taimi moni PCR aveina iʻuga ma maualuga lagona faʻaalia, lelei toe faʻaleleia ma lelei gradient dilution laina. Eseesega faʻalaʻeiʻau o viral viral nucleic acid faʻataʻitaʻiga e mafai ona iloa saʻo.
Taimi Post: Ape-11-2021
