Eleele maneta Ma nofoa Stool DNA

Pusa maneta mafai ona iloaina le maualuga-auala ma vave aveʻesega o genomic DNA mai le eleele / faʻataʻavalevale / usus microorganism.

O le pusa faʻaaogaina se tulaga faʻapitoa buffer faiga mafai ona aveʻese humic acid mai le eleele faʻataʻitaʻiga tele i le mafai. O loʻo iai ma tioata e mafai ona tuʻimomomoina lelei vaega eseese o le eleele faʻataʻitaʻiga ma mautinoa le faʻamaoni o le aveʻese mai le genomic DNA mai le palapala. E talafeagai foi mo le toʻesea mai o le faʻataʻitaʻi faʻataʻitaʻiga ma le manava microorganisms.

Pusi leai	Tele teuina
4992736	50 preps
4992738	200 preps

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Oloa Faʻamatalaga

Faʻataʻitaʻiga Faʻataʻitaʻiga

FAQ

Pine o oloa

Features

■ Lautele faʻaaogaina: E talafeagai mo le tosoina mai o ituaiga eseese o eleele faʻataʻitaʻi faʻataʻitaʻiga e pei o fugalaʻau palapala, eleele fugalaʻau, eleele faʻatoʻaga, mauga vaomatua eleele, palapala, palapala mumu, eleele uliuli, pefu ma isi. E talafeagai foi mo le toʻesea mai o le faʻataʻitaʻi faʻataʻitaʻiga ma le manava microorganisms.
■ Faigofie gaioiga: O le faʻataʻitaʻiga faʻagaioiga mafai ona maeʻa i se taimi puʻupuʻu.
■ Tulaga maualuga mama: Tuʻufaʻatasia ma maneta faʻamamaina faʻamamaluina, o le DNA maua mai i ai maualuga mama ma e mafai ona faʻaaogaina saʻo i lalo ifo o faʻataʻitaʻiga.

Talosaga

O le DNA faʻamamaina e le pusa o loʻo i ai ni mea leaga ma faʻamaoni lelei, ma e mafai ona faʻaaoga saʻo i isi faʻataʻitaʻiga i lalo ole biology o meaola pei ole PCR, digestion o le enzyme, ma isi.

Uma oloa mafai ona faʻapitoa mo ODM / OEM. Mo auiliiliga,faʻamolemole kiliki Customized Service (ODM / OEM)

Talu ai: Magnetic Serum / Plasma DNA Maxi Pusa

Sosoo ai: Maneta Faasalalauina DNA Maxi Pusa V2

	O le Genomic DNA na aumaia mai le 500 mg o le togalaʻau eleele e le luluina ma le olo olo faʻatasi ai ma le TIANGEN Magnetic Soil And Stool DNA Kit ma oloa talafeagai mai le Supplier M. 5 μl o le 100 μl eluate na uta i le 1% agarose gel electrophoresis. M: TIANGEN Tufuga D2000
	O le Genomic DNA na aumaia mai i nofoa o tagata ma TIANGEN Magnetic Soil And Stool DNA Kit ma oloa talafeagai mai le Supplier M, ma na mauaina e le PCR ma le 16S primers o siama. 5 μl o le 20 μl oloa PCR na utaina ile 1% agarose gel electrophoresis. M: TIANGEN Tufuga D2000
	O le Genomic DNA na aumaia mai iʻa iʻa microorganisms faʻaaogaina TIANGEN Magnetic Soil And Stool DNA Kit ma oloa talafeagai mai le Supplier M faʻatulagaina, ma na maitauina e PCR ma aoao masani 27F / 1492R o siama ma le oloa e tusa ma le 1500 bp. 5 μl o le 20 μl oloa PCR na utaina ile 1% agarose gel electrophoresis. M: TIANGEN Tufuga D2000

Q: Laʻititi pe leai foi DNA i le eluent.

A-1 Maualalo le totoina o sela poʻo siama i le amataga o le faʻataʻitaʻiga - Faʻamalosia le faʻasologa o sela poʻo siama.

A-2 Lysis le lava o faʻataʻitaʻiga - O faʻataʻitaʻiga e leʻi maeʻa maeʻa faʻafefiloi ma le buffer lysis. E fautuaina e fefiloi maeʻa ile pulse-vortexing mo le 1-2 taimi. —Ua le lava le sela o le sela na mafua mai i le faʻaititia o proteinase K. - Le lava le lysis sela poʻo le faʻaleagaina o le polotini ona o le le lava o le vai tavela mafanafana. E fautuaina e tipi le fasi pepa i ni fasi laiti ma faʻalautele le taimi taele e aveʻese uma toega i le lysate.

A-3 Le lava faʻasalalauga DNA. —E leai se ethanol poʻo se maualalo pasene nai lo le 100% ethanol na faʻaopopoina ae le i faʻamatuʻu le lysate i le vilivalavala.

A-4 O le taua ole pH ole faʻaola eletise e maualalo tele. —Faiaso le pH i le va o le 8.0-8.3.

Q: E leʻo lelei le faʻagaioiga ole DNA ile faʻataʻitaʻiga ole faʻasolosolo ole enzymatic.

Ethanol totoe i le eluent.

—E i ai toega fufuluina PW i le eluent. O le ethanol e mafai ona aveʻese i le centrifuging le vili pou mo 3-5 min, ona tuʻu ai lea i le potu vevela poʻo le 50 ℃ incubator mo 1-2 min.

Q: DNA faʻaleagaina

A-1 O le faʻataʻitaʻiga e le fou. — Aveʻese se faʻataʻitaʻiga lelei o le DNA e fai ma faʻatonutonu e faʻamautinoa ai pe ua faʻaleagaina le DNA i totonu o le faʻataʻitaʻiga.

A-2 Leaga muamua togafitiga. —Faʻailoaina e le soʻona suavaia oona nitrogen, toe maua le susu, poʻo tele tele o le faʻataʻitaʻiga.

Q: Faʻafefea ona faʻatinoina le muaʻi faʻaaliga mo le aveʻeseina o le gDNA?

E tatau ona fesuisuiaʻi mea e fai mo faʻataʻitaʻiga eseese. Mo faʻataʻitaʻiga laʻau, ia mautinoa ia olo lelei i le nitrogen suavaia. Mo manu faʻataʻitaʻiga, homogenate poʻo olo lelei i le suavaia oona. Mo faʻataʻitaʻiga ma sela sela e faigata ona gagau, pei o le G + bacteria ma le fefete, e fautuaina e faʻaoga le lysozyme, lyticase poʻo metotia e motusia ai sela o sela.

Q: O le a le eseesega i le va o le tolu laau gDNA aveʻesea pusa 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Laʻau Genomic DNA Kit faʻaaogaina se koluma-faʻavae metotia e manaʻomia chloroform mo le aveʻesea. E sili ona talafeagai mo eseese fualaʻau faʻataʻitaʻi, faʻapea foi ma laʻau mago paʻu. O le Hi-DNAsecure Plant Kit o loʻo faʻavaeina foi i luga ole koluma, ae leai se manaʻoga o le faʻateʻaina o le phenol / chloroform, e saogalemu ai ma e le oona foi. E fetaui lelei mo laʻau ma maualuga polysaccharides ma polyphenol mea. 4992709/4992710 DNAquick Plant System faʻaogaina se vai-faʻavae metotia. Phenol / chloroform aveʻesea e le manaʻomia foi. O le faʻamamaina gaioiga e faigofie ma vave ma leai se tapulaʻa mo le amataga amataga aofaʻiga, o lea e mafai ai e tagata faʻaoga ona fetuʻunaʻi le aofaʻi fetuʻunaʻi e tusa ai ma faʻataʻitaʻiga manaʻoga. Tele lapoa o gDNA fasi e mafai ona maua ma maualuga fua.

O le a le faʻatatauina maua o gDNA mai le 1 ml toto faʻataʻitaʻiga e TIANamp Toto DNA Pusa?

O le genomic DNA na aumaia mai vaega eseese o faʻataʻitaʻiga o le toto o le tagata e le TIANamp Blood DNA Kit. O iʻuga e mulimuli mai. O iʻuga e lisiina naʻo faʻasino, o le mea moni toʻeseʻesea iʻuga e faʻamoemoeina i tuutuuga o faʻataʻitaʻiga.

F: Mafai e le 4992207/4992208 ma le 4992722/4992723 ona faʻaaogaina e aveʻesea ai le toto masaa o le DNA?

E mafai ona faʻatino le vailaʻau toto DNA e faʻaaoga ai meaʻai na saunia i nei mea e lua i le naʻo le suia o le maliega faʻapitoa i faʻatonuga faʻapitoa mo le aveʻesea o le toto o le DNA. O le kopi vaivai o le toto clot DNA aveʻesega maliega maliega faʻapitoa mafai ona tuuina atu pe a talosagaina.

F: Pe a faʻaogaina le TIANamp Genomic DNA Kit, faʻafefea ona gagau ia mea fou i totonu o le sela taofia?

Taofi le faʻataʻitaʻiga fou ile 1 ml PBS, saline masani poʻo le buffer TE. Faʻamaeʻa uma homogenize le faʻataʻitaʻiga e se homogenizer ma ao le oso i lalo o le paipa e centrifuging. Tuʻu ese le supernatant, ma resuspend le oso i le 200 μl buffer GA. Ole faʻamamaina ole DNA e mafai ona faia e tusa ma faʻatonuga.

Q: Faʻafefea ona filifili le oloa mo le DNA toʻesea mai plasma, serum ma tino faʻataʻitaʻiga suavai?

Mo le faʻamamaina o gDNA i plasma, serum ma tino faʻataʻitaʻiga suavai, TIANamp Micro DNA Pusa ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum / faʻataʻitaʻiga plasma, TIANamp Virus DNA / RNA Kit ua fautuaina. Mo le faʻamamaina o siama gDNA mai serum ma faʻataʻitaʻiga plasma, TIANamp Bacteria DNA Pusa ua fautuaina (lysozyme tatau ona aofia ai mo lelei siama). Mo faʻataʻitaʻiga faua, Hi-Swab DNA Pusa ma TIANamp Bacteria DNA Pusa ua fautuaina.

Q: Faʻafefea ona filifili ato mo le aveʻesea o le gDNA mai faʻataʻitaʻiga mai le fungi?

DNAsecure Plant Pusa poʻo DNAquick Plant System e fautuaina mo le aveʻese mai o le genome. Mo le toʻafilemuina o le genome, TIANamp Yeast DNA Kit e fautuaina (lyticase tatau ona saunia e ia lava).

Pusa E leai se faʻaaogaina Dna Aveʻiga Pusa