FastKing One Step RT-qPCR pusa

Tasi-sitepu Moni-Taimi RT-PCR ma SYBR-Green.

FastKing One Step RT-qPCR Kit ua fuafuaina mo Real-Time One Step RT-qPCR e ala i le faʻaaogaina o le SYBR Green I. O le kit e mafai ona faʻaaogaina i le taimi moni i le tasi sitepu RT-qPCR o RNA taulaʻiga, ma fesuiaʻi tusitusiga ma PCR o loʻo faia faʻasologa. i le paipa tutusa. O le mea lea e faigofie ai le faʻagaioiga, ma e faʻaititia ai le aʻafiaga o le faʻaleagaina.

O le 25 × RT-PCR Enzyme Mix i lenei pusa o se faʻailoga o le tusi tala i tua a le TIANGEN (FastKing RTase), o le antibody Taq DNA polymerase ma RNase Inhibitor. O le FastKing RTase o se ituaiga fou o mole mole fesuiaʻiga o suiga o transcriptase. Faatasi ai ma le faʻaopopoina faʻaopopoina hydrophobic motif, lea e sili atu le malosi RNA vavalalata ma vevela mautu. O le FastKing RTase ua faʻaleleia le toe faʻafouina o tusitusiga ma le agavaʻa e faʻalauteleina le RNA template ma laʻasaga lona lua. Le ituaiga fou o le vevelaTaq DNA polymerase ma sili ona lelei gaioiga o loʻo faʻaaogaina i le PCR gaioiga, faia ai le PCR tali atu ina ua maeʻa faʻaliliuga o loʻo maualuga le faʻamalosia lelei ma faʻapitoa. I se faʻaopopoga, o le 2 × FastKing RT-qPCR Buffer (SYBR Green) i lenei oloa o se fou fou tali faʻapitoa faʻapitoa faʻaleleia mo le sili atu lua ki enzymes, e aofia ai talafeagai ionic vaega, dNTPs, SYBR Green vali, tasi laasaga stabilizer ma enhancer, lea mafai ona mautinoa o FastKing RTase ma le fou vevela amataTaq DNA polymerase mafai ona faia le sili ona aoga matafaioi i le atoa tasi-laasaga tali atu gaioiga.

Lenei pusa faʻaavanoa lelei faʻamau faʻailoga i se lautele lautele o aofaʻiga eria. E mafai ona faʻamaoni ma fuainumera faʻailogaina le tele o maualuga ma maualalo tele gaioiga genes ma lelei toe faʻafouina ma maualuga faʻatuatuaina.